The non-detergent solubilization and isolation of intracytoplasmic membrane polypeptides from Rhodopseudomonas sphaeroides.
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چکیده
منابع مشابه
Solubilization, isolation, and immunochemical characterization of the major outer membrane protein from Rhodopseudomonas sphaeroides.
Solubilization of the major outer membrane protein of Rhodopseudomonas sphaeroides, and subsequent isolation, has been achieved by both non-detergent- and detergent-based methods. The protein was differentially solubilized from other outer membrane proteins in 5 M guanidine thiocyanate which was exchanged by dialysis for 7 M urea. The urea-soluble protein was purified to homogeneity by a combin...
متن کاملEffect of heat and 2-mercaptoethanol on intracytoplasmic membrane polypeptides of Rhodopseudomonas sphaeroides.
Solubilization at 75 degrees C of Rhodopseudomonas sphaeroides chromatophores in the presence of sodium dodecyl sulfate (SDS) and 2-mercaptoethanol (beta-ME) resulted in the selective absence of reaction center B and C polypeptides from SDS-polyacrylamide gel electrophoresis profiles. A newly identified, chromatophore-specific polypeptide, with a mass of 35.2 kdaltons, was also missing under th...
متن کاملIntracytoplasmic membrane synthesis in synchronous cell populations of Rhodopseudomonas sphaeroides. Polypeptide insertion into growing membrane.
A nonspecific density labeling technique has been employed to monitor the synthesis of intracytoplasmic membrane in synchronously dividing populations of Rhodopseudomonas sphaeroides. The intracytoplasmic membranes of cells synchronized in D2O-based medium were found to undergo discontinuous decreases in specific density during synchronous cell growth following transfer to H2O-based medium. The...
متن کاملThe relationship of intracytoplasmic membrane assembly to the cell division cycle in Rhodopseudomonas sphaeroides.
Discontinuous increases in the net accumulation of cellular phospholipid were previously observed during the synchronous growth of Rhodopseudomonas sphaeroides (Lueking, D. R., Fraley, R. T., and Kaplan, S. (1978) J. Biol. Chem. 253, 451-457). This pattern is shown to result from large increases in the rate of phospholipid synthesis, occurring just prior to cell division. The absence of detecta...
متن کاملHeat modifiability and detergent solubility of outer membrane proteins of Rhodopseudomonas sphaeroides.
The outer membrane fraction from Rhodopseudomonas sphaeroides was isolated by isopycnic density centrifugation. The purity of this fraction was assayed by several methods. When the outer membrane fraction obtained after French press lysis of cells was compared with the outer membrane fragments released during spheroplast formation, the polypeptide profiles were identical. Detergent solubilizati...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1981
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)69294-x